Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.

Nuclear transport in vitro.

In this paper, progress towards the goal of understanding communication between the nucleus and cytoplasm using an in vitro system is reviewed. To probe the mechanism of nuclear targeting, we developed an in vitro transport system and have begun to dissect the highly selective process of nuclear transport. The basic parameters of transport were defined using an easily isolated nuclear protein, ...

In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins

An in vitro system was developed that provides a quick microscopic assay for nuclear transport. The assay uses an extract of Xenopus eggs, normal or synthetic nuclei, and a fluorescently labeled nuclear protein, nucleoplasmin. This in vitro system accurately mimics in vivo nuclear transport, both in exclusivity and in the amount of accumulation observed (up to 17-fold). Selective accumulation o...

features of short story in translated and non-translated literature: a polysystemic perspective

ترجمه متون ادبی در دنیای امروز از اهمیت ویژه ای برخوردار است. فرهنگ های مختلف با استفاده از متون ادبی ملل و فرهنگ های دیگر سعی در غنی کردن فرهنگ و ادبیات کشور خود دارند. کشور ما نیز از این قاعده مستثنی نیست. در ایران، ترجمه متون ادبی اروپائی که با دوران مشروطه ایرانی آغاز می شود موجب تغییرات اساسی در فرهنگ، شیوه زندگی و تفکر ایرانیان شده است. در این دوران نه تنها در امورات سیاسی و فکری کشور تغی...

In vitro proteolytic processing of a diubiquitin and a truncated diubiquitin formed from in vitro-generated mRNAs.

Ubiquitin, a highly conserved protein of 76 amino acids found in all eukaryotes, is translated from mRNAs that contain either multiple, contiguous coding sequences of the protein or a single ubiquitin coding sequence fused to sequences coding for 52 or 76 amino acids. We describe here formation of monoubiquitin from in vitro translation of mRNAs containing either two complete sequences or one c...

Numerical Simulation of Transport Phenomena in Nuclear Cores